Characterization and expansion mechanisms of human iPS cell-derived hepatic progenitor-like cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860529
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokai University
• Principal Investigator: TSURUYA Kota 東海大学, 医学部, 助教 (00725377)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: iPS細胞 / 肝前駆細胞 / 細胞表面分子

Outline of Final Research Achievements

Hepatic stem/progenitor cells in liver development have a high proliferative potential and the ability to differentiate into both hepatocytes and cholangiocytes. In this study, we focused on the cell surface molecules of human induced pluripotent stem(iPS) cell-derived hepatic progenitor-like cells and analyzed how these molecules modulate expansion of these cells. Human iPS cells were differentiated into immature hepatic lineage cells by cytokines. In addition to hepatic progenitor markers (CD13 and CD133), the cells were co-immunostained for various cell surface markers and analyzed.
This study revealed the expression profiles of cell surface molecules in CD13+CD133+ cells derived from human iPS cells. Moreover, IGF-1R, EGFR, erbB2, and Fn14 were highly expressed, and IGF and TWEAK were important for proliferation in CD13+CD133+ cells derived from human iPS cells.

Free Research Field

消化器内科学