2016 Fiscal Year Final Research Report
Role of Ape1 on Capillary Stem Cells
| Project/Area Number |
26860540
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 組織幹細胞 / 酸化ストレス / 再生医療 |
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| Outline of Final Research Achievements |
In order to develop the method to improve Cell transplantation therapy using Ape1 gene, we performed a series of Ape1 loss/gain function study. Ape1-recombinant adenovirus was used for Ape1-overexpression and Ape-specific siRNA was used for knockdown of endogenous Ape1 in target stem cells, i.e., capillary stem cells and cardiac stem cells. We found that Ape1 protected these cells from damage of oxidative stress and increased the survival rate after transplantation into ischemic tissues. Transplantation of Ape1-expressed Sca1-positive cardiac stem cells (CSC) into ischemic/reperfusion mouse heart significantly improved cardiac function compared to control CSC.
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| Free Research Field |
循環器内科
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