2015 Fiscal Year Final Research Report
Impact of clinical characteristics on the yield of therapeutic adipose-derived mesenchymal stem cells in a cardiovascular disease
| Project/Area Number |
26860549
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Oto 金沢大学, 附属病院循環器内科, 医員 (00781147)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 間葉系幹細胞 / 皮下脂肪 / 心血管疾患 |
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| Outline of Final Research Achievements |
The problem of autologous stem cell therapy for cardiovascular disease is that a large variability of therapeutic effects among patients. We conducted a clinical study to examine the variation in yield of therapeutic stem cells in subcutaneous adipose tissue (SAT) among patients. From 17 patients who underwent cardiac surgery, 1-2g of SAT was obtained and digested with collagenase to isolate stromal vascular fraction (SVF). Mesenchymal stem cell markers in SVF were analyzed by flow cytometry. The frequency of the patients with hypertension, dyslipidemia and diabetes was 94%, 77% and 59%, respectively. Interestingly, the content of CD271-positive cells that is reported as a specific marker of therapeutic stem cells largely varied among patients and was associated with the specific clinical feature. These indicated that the clinical background would impact on yield of therapeutic stem cells in SAT and efficacy of cardiac stem cell therapy.
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| Free Research Field |
再生治療
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