2015 Fiscal Year Final Research Report
The use of human pluripotent stem-cell-derived renal/vascular precursors and organ decellularization for the generation of a kidney organoid
| Project/Area Number |
26860643
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 腎臓再生 / 尿細管 / ヒト多能性幹細胞 |
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| Outline of Final Research Achievements |
Methods to purify specific types of cells from differentiated human pluripotent stem cells (hPSCs) would be useful to obtain pure populations of specific types of kidney cells. We report a simple two-step differentiation protocol to generate kidney tubular organoids from hPSCs with direct purification of KSP-positive cells using anti-KSP antibody.
We differentiated hPSCs into mesoderm cells using a glycogen synthase kinase-3βinhibitor, subsequently cultured cells in renal epithelial growth medium to induce KSP+ cells. We purified KSP+ cells using flow cytometry with anti-KSP antibody, and cultured KSP+ cells in 3D Matrigel, which formed tubular organoids in vitro. KSP+ cells also allowed for the generation of chimeric kidney cultures in which human cells self-assembled into 3D tubular structures in combination with mouse embryonic kidney cells. This approach is useful for generating cells of kidney tubular lineage cells that would be usable as a source for kidney regeneration.
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| Free Research Field |
腎臓再生
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