2015 Fiscal Year Final Research Report
Investigating the pathomechanism underlying idiopathic Parkinson's disease using patient-derived iPS cells
| Project/Area Number |
26860666
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Kyoto University |
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Principal Investigator |
Yamakado Hodaka 京都大学, 医学(系)研究科(研究院), 助教 (10378771)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | パーキンソン病 |
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| Outline of Final Research Achievements |
Genomic sequencing was performed to identify GBA-related Parkinson's disease patients, and five clones of iPS cells from 6 patients were generated by plasmid methods. Genome editing by CRISPR/cas9 was employed to edit GBA gene (L444), but its efficiency was about 10%, and it was lower than expected, even in HEK 293 cells. To overcome the disadvantages from heterogeneity of genetic background in iPS research, we are analyzing the corin-sorted dopaminergic precursor cells, as well as increasing the number of target clones.
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| Free Research Field |
パーキンソン病
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