Lipidomic analysis of metabolic syndrome : Development of a sensitive liquid chromatography-mass spectrometry method for triacylglycerol

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860686
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Akita University
• Principal Investigator: Noriko Ueno 秋田大学, 学内共同利用施設等, 研究員 (90546631)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: トリアシルグリセロール / 質量分析 / リピドミクス

Outline of Final Research Achievements

High levels of triacylglycerols (TAG) in the blood are associated with risk of the metabolic syndrome. It is hard to do the quantitative analysis of TAG molecular species using currently available methods, although it can apply to the quantitative analysis of total TAG and/or its subclass. In this study, I developed a novel analysis method, which is a liquid chromatography-mass spectrometry (LC-MS) for the high separative, high sensitive and qualitative TAG analysis. Furthermore, this method is able to also measure many lipid metabolites such as phospholipid and cholesteryl ester, diacylglycerol. In future, I aim at the elucidation of a biological phenomenon by global and/or specific comprehension of biological lipids using established method.

Free Research Field

脂質生化学　脂質分析学