2016 Fiscal Year Final Research Report
Systematic analysis of megakaryopoiesis and platelet function
| Project/Area Number |
26860718
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 巨核球 / 血小板 |
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| Outline of Final Research Achievements |
Megakaryocytes (MKs) differentiate from hematopoietic stem cells under the control of MK-specific cytokine, thrombopoietin (TPO). Subsequent maturation of MKs is morphologically characterized by polyploidization and expansion of the cytoplasmic body. Final stage of differentiation is characterized by platelet release from the ends of long thin cytoplasmic processes called proplatelet. However, the mechanism of proplatelet formation and platelet function have not been completely understood.
In this study, proplatelet formation, MK ploidy and platelet activation including platelet aggregation of dnam-1, calpastatin KO platelets were changed comparing with those of wild-type. These results suggest that DNAM-1, calpain-calpastatin are involved in megakaryopoiesis and platelet function. Furthermore, we demonstrated that sodium bicarbonate facilitates hemostasis.
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| Free Research Field |
血栓・止血学
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