2016 Fiscal Year Final Research Report
Functional significance of the expression of CD34 antigen on the human hematopoietic stem cells
| Project/Area Number |
26860741
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 造血幹細胞 / CD34抗原 |
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| Outline of Final Research Achievements |
The hematopoietic stem cells (HSCs) possess both self-renewal and multi-lineage differentiation abilities and maintain lifelong hematopoiesis. It is generally considered that all of human HSC exist in the CD34-positive fraction. However, it is not clarified that the functional significance of the expression of CD34 antigen on the human hematopoietic stem cells. In order to elucidate this problem, I tried to the knock-out (KO) of CD34 antigen from human HSCs using CRISPR/Cas9 system. In the results, I succeeded to KO CD34 antigen from human HSC, though the frequency of KO was slightly low. Therefore, it is necessary to improve the efficiency of CD34 antigen KO system. The modification of CD34 KO system is now ongoing.
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| Free Research Field |
幹細胞生物学
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