Establishment of a lupus animal model by SKG mice on C57BL/6 background

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860749
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Kyoto University
• Principal Investigator: Hashimoto Motomu 京都大学, 医学(系)研究科(研究院), 助教 (60512845)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 全身性エリテマトーデス / 動物モデル / CD4 T細胞 / T細胞レセプターシグナル / Toll like receptor

Outline of Final Research Achievements

SKG mouse on BALB/c background is a murine model for rheumatoid arthritis. We backcrossed SKG mice into C57BL/6 background to generate SKG mice on C57BL/c background (C57BL/6 ZAP70skg/skg mice; B6 SKG mice). B6 SKG mice developed lupus like nephritis with immune complex deposition and anti-DNA antibody production after chronic stimulation with Toll like receptors agonists. In B6 SKG mice, follicular helper T cells (CXCR5+, PD-1high, Bcl6+) were spontaneously expanded and helped forming the germinal center B cells (Fas+, GL-7+) to produce anti-DNA antibody.

Free Research Field

医歯薬学　内科系臨床医学　膠原病・アレルギー内科学