Does the prognosis of ventilator-associated pneumonia (VAP) affected by innate immune response in the lung?

2017 Fiscal Year Annual Research Report

• Project/Area Number: 26860778
• Research Institution: Department of Clinical Research, National Hospital Organization Nagasaki Medical Center
• Principal Investigator: 山本 和子 独立行政法人国立病院機構(長崎医療センター臨床研究センター), 臨床研究センター, 客員研究員 (10398167)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 人工呼吸器関連肺炎 / 自然免疫 / 肺上皮細胞 / 予後因子 / サイトカイン

Outline of Annual Research Achievements

１. immuno PCRを用いた高感度のサイトカイン測定
集積した人工呼吸器関連肺炎の気管支肺胞洗浄液サンプルで、平成28年11月/12月にELISA測定したサイトカインGM-CSFとIL-1αに関して、大部分の検体において測定感度以下であったため、より測定感度の高いimmuno PCRを用いて測定を行った。検体数は当初の目標である50検体に達し、CXCL5, GM-CSF, CCL20, IL-1α, IL-1β, TNF-α, IL-6, IL-8の測定を行った。現在患者背景因子と予後項目（６０日以内死亡、ICU入院期間、全入院期間、人工呼吸器装着期間）に対する各種サイトカインの関連性と、サイトカインの組み合わせによる予後項目との関連性について解析中である。
２. 本研究に関連して、以下の原著論文を報告した。
(1) Smith NMS, Yamamoto K, Mizgerd JP, et al. Regionally compartmentalized resident memory T cells mediate naturally acquired protection against pneumococcal pneumonia. Mucosal Immun 11:220-235, 2018. (2) Wasserman GA, Yamamoto K, Jones MR, et al.Expression of Piwi protein MIWI2 defines a distinct population of multicilliated cells. J Clin Invest 127:3866-3876, 2017. (3) Coleman FT, Yamamoto K, Mizgerd JP, et al. The capacity of pneumococci to activate macrophage NF-kB determines necroptosis and pneumonia severity. J Infect Dis 216:425-435, 2017.
３. 本研究に関連して、以下の学会発表を行った。
Yamamoto K. The impact of neutrophil recruitment in mediastinal lymph node to invasive pneumococcal pneumonia. 第58回 日本呼吸器学会学術講演会 (大阪)呼吸器領域若手グローバル研究者シンポジウム 2018年4月27日

Research Products

• [Int'l Joint Research] Boston University School of Medicine(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: Boston University School of Medicine
• [Journal Article] Examination of cryptococcal glucuronoxylomannan antigen in bronchoalveolar lavage fluid for diagnosing pulmonary cryptococcosis in HIV-negative patients. (2018)
  • Author(s): Oshima K, Takazono T, Saijo T, Tashiro M, Kurihara S, Yamamoto K, Imamura Y, Miyazaki T, Tsukamoto M, Yanagihara K, Mukae H, Kohno S, Izumikawa K
  • Journal Title: Medical Mycology Volume: 56 Pages: 88-94
  • DOI: 10.1093/mmy/myx010
  • Peer Reviewed
• [Journal Article] Regionally compartmentalized resident memory T cells mediate naturally acquired protection against pneumococcal pneumonia. (2018)
  • Author(s): Smith NMS, WassermanGA, Coleman FT, Hilliard KL, Yamamoto K, Lipsitz E, Malley R, Dooms H, Jones MR, Quinton LJ, Mizgerd JP
  • Journal Title: Mucosal Immunology Volume: 11 Pages: 220-235
  • DOI: 10.1038/mi.2017.43
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Adjunctive corticosteroid therapy for inpatients with Mycoplasma pneumoniae pneumonia. (2017)
  • Author(s): Tashiro M, Fushimi K, Kawano K, Takazono T, Saijo T, Yamamoto K, Kurihara S, Imamura Y, Miyazaki T, Yanagihara K, Mukae H, Izumikawa K
  • Journal Title: BMC Pulmonary Medicine Volume: 17 Pages: 219-219
  • DOI: 10.1093/mmy/myx010
  • Peer Reviewed
• [Journal Article] Expression of Piwi protein MIWI2 defines a distinct population of multicilliated cells. (2017)
  • Author(s): Wasserman GA, Szymaniak AD, Hinds AC, Yamamoto K, Kamata H, Smith NMS, Hilliard KL, Carrieri C, Labadorf AT, Quinton LJ, Ai X, Varelas X, Chen F, Mizgerd JP, Fine A, O’Carroll D, Jones MR.
  • Journal Title: Journal of Clinical Investigation Volume: 127 Pages: 3866-3876
  • DOI: 10.1172/JCI94639
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The capacity of pneumococci to activate macrophage NF-kB determines necroptosis and pneumonia severity. (2017)
  • Author(s): Coleman FT, Blahna MT, Kamata H, Yamamoto K, Zabinski MC, Kramnik I, Wilson AA, Kotton DN, Quinton LJ, Jones MR, Pelton SI, Mizgerd JP
  • Journal Title: Journal of Infectious Diseases Volume: 216 Pages: 425-435
  • DOI: 10.1093/infdis/jix159
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] In vitro and In vivo antifungal activities of T-2307, a novel arylamidine, against Cryptococcus gatii: an emerging fungal pathogen (2017)
  • Author(s): Nishikawa H, Fukuda Y, Mitsuyama J, Tashiro M, Tanaka A, Takazono T, Saijo T, Yamamoto K, Nakamura S, Imamura Y, Miyazaki T, Kakeya H, Yamamoto Y, Yanagihara K, Mukae H, Kohno S, Izumikawa K.
  • Journal Title: Journal of Antimicrobial Agents and Chemotherapy Volume: 72 Pages: 1709-1713
  • DOI: 10.1093/jac/dkx020
  • Peer Reviewed
• [Journal Article] Comparison of efficacy of antimicrobial agents among hospitalized patients with Mycoplasma pneumoniae pneumonia in Japan during large epidemics of macrolide-resistant M.pneumoniae infections: A nationwide observational study. (2017)
  • Author(s): Tashiro M, Fushimi K, Kawano K, Takazono T, Saijo T, Yamamoto K, Kurihara S, Imamura S, Miyazaki T, Yanagihara K, Mukae H, Izumikawa K.
  • Journal Title: Clinical Infectious Diseases Volume: 65 Pages: 1837-1842
  • DOI: 10.1093/cid/cix695
  • Peer Reviewed
• [Presentation] The capacity of pneumococcus to stimulate macrophage NF-kappa B activity dictates the seveerity of adult pneumococcal pneumonia (2018)
  • Author(s): Kazuko Yamamoto, Shuhei Ideguchi, Shotaro Ide, Takahiro Takazono, Tomomi Saijo, Yoshifumi Imamura, Taiga Miyazaki, Katsunori Yanagihara, Yuichi Fukuda, Bin Chang, Fadie T. Coleman, Joseph P. Mizgerd, Hiroshi Mukae
  • Organizer: Gordon Research Conference 2018, Biology of Acute Respiratory Infection
• [Presentation] The impact of neutrophil recruitment in mediastinal lymph node to invasive pneumococcal pneumonia (2018)
  • Author(s): 山本 和子
  • Organizer: 第58回日本呼吸器学会学術講演会
• [Presentation] 縦隔リンパ節内好中球による肺炎球菌肺炎の侵襲性感染症の防御 (2017)
  • Author(s): 山本 和子, 田中 章貴, 田代 将人, 西條 知見, 今村 圭文, 宮崎 泰可, 泉川 公一, 迎 寛
  • Organizer: 第57回日本呼吸器学会学術講演会
• [Presentation] 当科における膿胸の治療経過に関連する患者背景因子の検討 (2017)
  • Author(s): 井手口 周平, 山本 和子, 井手 昇太郎, 田中 章貴, 田代 将人, 小佐井 康介, 賀来 敬仁, 西條 知見, 今村 圭文, 宮崎 泰 可, 泉川 公一, 柳原 克紀, 迎 寛
  • Organizer: 第91回日本感染症学会総会
• [Presentation] Significance of antimicrobial treatment-related factors for the outcomes of adult empyema in Japanese tertiary hospitals (2017)
  • Author(s): Daisuke Okuno, Shuhei Ideguchi, Kazuko Yamamoto, Tomomi Saijo, Yoshifumi Imamura, Taiga Miyazaki, Naoki Iwanaga, Yuichi Fukuda, Kazuhiro Yatera, Hiroshi Mukae
  • Organizer: 30th International Congress of Chemotherapy and Infection 2017
  • Int'l Joint Research