2017 Fiscal Year Final Research Report
Augmentation of the cytotoxicity of NK cells by IL-21 gene tansduction
| Project/Area Number |
26860785
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Niigata University |
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Principal Investigator |
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| Research Collaborator |
KASAHARA Yasushi
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 細胞療法 / NK細胞 / 遺伝子導入 / 小児悪性腫瘍 / サイトカイン |
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| Outline of Final Research Achievements |
We transferred the IL-21 gene into primary NK cells with retroviral vector. The both membrane-binding and secretory type of IL-21 retroviral vectors were constructed. Average transduction efficiencies of IL-21 genes were over 90%. The cytotoxicity of gene-transduced NK cells against K562 and Jurkat cell lines was assessed by four-hour and 72-hour co-culture assay, which revealed augmentation of the cytotoxicity of IL-21 gene transferred NK cells. We confirmed the elevation of IL-21 expression and perforin and granzyme B production in gene-transferred NK cells. That may be one of the reasons of cytotoxicity augmentation. NK cell life span did not extend by both membrane-binding and secretory type of IL-21 gene transduction. While the cytotoxicity of the membrane-binding type would not acheive that of the secretory type, there was no significant difference for the cytotoxicity. The membrane-binding type is expected to reduce cytokine-release symptoms.
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| Free Research Field |
小児悪性腫瘍
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