2016 Fiscal Year Final Research Report
Analysis of the interaction between Merkel cell carcinoma and squamous cell carcinoma in collagen gel matrix culture.
| Project/Area Number |
26860884
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | メルケル細胞癌 / メルケル細胞ポリオーマウイルス / 有棘細胞癌 / 混合培養 |
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| Outline of Final Research Achievements |
Merkel cell carcinoma (MCC) is a highly aggressive skin cancer linked to a contributory virus, Merkel cell polyomavirus (MCPyV). MCPyV DNA has been confirmed to be present in approximately 80% of MCCs. We had several extremely rare cases of MCC combined with Squamous cell carcinoma (SCC). All reported MCC combined with SCC were MCPyV-negative. Thus, we hypothesized that there are differences of the cell characteristics between MCPyV-positive and -negative MCCs in a coexistence environment with SCC. SCC cell line, DJM-1, inhibits proliferation of MCPyV-positive MCC cell line, MKL-1 cells, but not MCPyV-negative cell lines in collagen gel matrix culture. DJM-1 cells induce apoptosis of MKL-1 cells. MKL-1 cells inhibit proliferation of DJM-1 cells. This culture system shows the difference of biological behavior between MCPyV-positive MCC and MCPyV-negative MCC. It remains possible that MCPyV-positive and -negative MCC may develop through different tumorigenic pathways.
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| Free Research Field |
メルケル細胞癌
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