Significance of KEAP1 mutation in melnaoma cells.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860891
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Iwate Medical University
• Principal Investigator: Miura Shinpei 岩手医科大学, 医学部, 助教 (30713226)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: KEAP1 / NRF2 / CDDP / ドライバー変異

Outline of Final Research Achievements

We identified KEAP1 inactivation mutation in booth malignant melanoma cell lines and primary tumors. Mutations of the KEAP1 genes related to drug resistance （CDDP and dacarbazine）. The ratio of reduced-to-oxidized glutathione (GSH/GSSG) was decreased by treatment with CDDP or DTIC in siNRF2-transfected cells. However, the mutation did not confer the tumorigenicty for xenografts. Theses results suggest that EAP/NRF2 inactivation could not be a driver mutation in melanoma cells.

Free Research Field

皮膚科学