Hereditary consideration of autoimmune disease related gene AIRE on pemphigus diseases and pemphigus -like diseases.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860905
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kyushu University (2015); Kurume University (2014)
• Principal Investigator: NISHIKAWA Ryuhei 九州大学, (連合)農学研究科(研究院), 学術研究員 (30534531)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: Early endosome antigen 1 / EEA1 / 天疱瘡 / 自己免疫疾患 / BIOCHIP / auto-antibody / pemphigus disease / autoimmune disease

Outline of Final Research Achievements

The major antigens of typical pemphigus disease are desmoglein, desmocollins and a plakin proteins. However, there are novel antigen supposed to be studied to classify the pemphigus disease patients. We used immunoblotting using a serum from an atypical autoimmune bullous disease patient to detect autoantibody, which react with unknown 175 kDa protein. Subsequent studies using two-dimensional gel electrophoresis, immunoblotting and mass-spectrometry identified 175 kDa protein as early endosome antigen 1 (EEA1). This finding was confirmed by subsequent immunological studies, including immunofluorescence of skin and cultured keratinocyte and two-dimensional gel immunoblotting with anti-EEA1 monoclonal antibody and absorption studies using EEA1 recombinant protein. We also developed a novel BIOCHIP assay using full length EEA1 cDNA to detect anti-EEA1 antibodies. However, none of 35 pemphigus sera showed anti-EEA1 antibodies in the BIOCHIP assay except the serum from index case patient.

Free Research Field

医学（皮膚科学）