2016 Fiscal Year Final Research Report
The role of the microRNA in the radiation resistance using pancreatic cancer cell
| Project/Area Number |
26860978
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 放射線抵抗性 / 膵癌 / microRNA |
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| Outline of Final Research Achievements |
First of all, we made a cell line that show radiation resitance by repeating low dose Gemcitabine exposure. We use a pancreatic cancer cell line with name of Panc-1. We name the radiation resistance cell line as Panc-1 XR. We compair the microRNA status of Panc-1 XR and wild type. Then try temporaly insertion of some kind of microRNA. Finally we made a cell line that stably overexpress the mir-34a by transecting vector. The stable OE cell line (panc-1-34aOE) show radiation sensibility against X-ray irradiation. Therefore, approaching to the microRNA mir-34a could improve the resistance against treatment in the pancreatic cancer.
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| Free Research Field |
放射線生物学
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