Understanding the brain pathology with individual immune cell tracking using high-field MRI scanner

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860996
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Osaka University
• Principal Investigator: Mori Yuki 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (10559355)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 磁気共鳴画像法 / 免疫細胞動態 / 細胞追跡 / 神経免疫

Outline of Final Research Achievements

We established highly sensitive in-vivo imaging techniques for sequential monitoring of intrinsic monocytes/macrophages migration in the mouse brain at the single-cell level by using 11.7T high-field magnetic resonance imaging (MRI) scanner. We have successfully improved the spatio-temporal resolution of MRI for cellular tracking and created a 4D cellular tracking dataset of intrinsic monocytes/macrophages in the living mouse brain. In addition, we conducted quantitative analysis of individual cells using this dataset. In this procedure, we found a significant difference in velocity and migration patterns between the contralateral and the ipsilateral cortex in the mouse stroke model.

Free Research Field

生体機能イメージング