Development of immunotherapy targeting regulatory B cell for gastrointestinal cancer.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Fukushima Medical University
• Principal Investigator: Saze Zenichiro 福島県立医科大学, 医学部, 助教 (10468126)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: MDSC / 制御性B細胞

Outline of Final Research Achievements

To verify whether low-density neutrophil cells have regulatory potential, we divided human PBMCs into low-density neutrophil cells and high-density cells. Then, we extracted CD15+ cells from each fraction, co-cultured with PBMCs under stimulation and measured INF-γ by ELISA. Suppression of INF-γ production was not observed in co-culture with high-density neutrophil cells. We were not able to examine suppression assay of co-culture with low-density neutrophil cells due to purity of CD15+ cells was low and the numbers of harvested low-density neutrophil cells from cancer patients were so small. Now we are trying to improve purity and quantity of low-density neutrophil cells. Using IHC, we analyzed correlation between CD15+ neutrophil in tumor and clinicopathological factors. No. of CD15+ cells of ly+ group was significantly higher than those of ly- group. We have plans to study about regulatory B cells from gastrointestinal cancer patients’ PBMC and tumor infiltrating lymphocytes.

Free Research Field

消化器外科