2016 Fiscal Year Final Research Report
A novel strategy for ubiquitin-fusion gene transduced dendritic cell vaccine in the control of the constituents of the proteasome
| Project/Area Number |
26861095
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | プロテアソーム / メソセリン / 樹状細胞ワクチン / ユビキチン |
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| Outline of Final Research Achievements |
Immature dendritic cells contains the constitutive proteasome, however, mature dendritic cells differentiated from immature dendritic cells exposed with inflammatory mediator such as interferon-gamma contains immunoproteasome (ip) derived from immature dendritic cells. The inhibition of the immunoproteasome in mature dendritic cells using small interfering RNA transfection targeting the 3 inducible ip subunits might help to induce antigen-specific cytotoxic T lymphocytes. In this study, I performed basic research about anti-tumor immune response targeting mesothelin which is tumor associated antigen of pancreatic ductal carcinoma, and using dendritic cells transduced with mesothelin gene fused with ubiquitin gene which is a degradation signal of proteasome.
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| Free Research Field |
膵癌免疫療法
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