Functional analysis of fuctional RNA involvement in colorectal cancer development and progression

2017 Fiscal Year Final Research Report

• Project/Area Number: 26861104
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Juntendo University
• Principal Investigator: TAKAHASHI Rina 順天堂大学, 医学部, 非常勤助手 (80639112)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: メチルトランスフェラーゼ / non-coding RNA / エピジェネティクス / 大腸癌 / 機能性RNA

Outline of Final Research Achievements

SMYD3 plays a crucial role in carcinogenesis. The intronic SMYD3 RNAis excised in intron of SMYD3 gene and control the expression of SMYD3. The role of intronic SMYD3 in colorectal cancer is largely unknown. To uncover the function of intronic SMYD3 RNA, in situ hybridization for intronic SMYD3 RNA were carried out using tissue microarray constructed from colorectal cancer samples. As a result, there was a decrease trend in its expression in metastatic colorectal cancer. Then I tried to establish the colorectal cancer cell lines which deleted intronic SMYD3 RNA. In further study, I will investigate whether intronic SMYD3 RNA are involved in metastasis or not using model organism and cancer cell lines.

Free Research Field

消化器外科学