2015 Fiscal Year Final Research Report
Scaffold-free tissue-engineered allogenic adipose-derived stem cells promote meniscus regeneration
| Project/Area Number |
26861177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 半月板再生 / 脂肪由来幹細胞 / 細胞凝集塊 / スフェロイド |
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| Outline of Final Research Achievements |
Purpose: To determine whether meniscal tissue could be regenerated by the implantation of adipose-derived stem cells (ADSCs) engrafted into the meniscal defect in a rabbit model of partial meniscectomy. Methods: We created full-thickness meniscal defects in the rabbit’s knee. The defects were left empty in the control group, and were filled with ADSCs which created a 3-dimensional structure of ADSCs in the ADSC group. Results: Macroscopically, full-thickness defects remained in the control group, however, the defects were not observed in any time point in the ADSC group. Histologically, there was a consistent tendency toward significantly superior tissue quality scores in the ADSC group compared with the control group at all weeks (p<.005). Regenerated tissue was positive for type I collagen and the extent of staining increased gradually over time in the ADSC group. Conclusions: This study shows that ADSCs adhere to meniscal defect and promote meniscus regeneration.
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| Free Research Field |
医歯薬学
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