Investigation of vertebral compression fracture healing methods

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861209
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kitasato University
• Principal Investigator: Saito Wataru 北里大学, 医学部, 助教 (60439099)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 椎体骨折 / 塩基性線維芽細胞増殖因子 / コラーゲン結合ドメイン / コラーゲン結合型成長因子

Outline of Final Research Achievements

We previously constructed a fusion protein consisting (CB-bFGF) of bFGF and the collagen binding domain (CBD) of Clostridium histolyticum collagenase and revealed that CB-bFGF markedly enhances bone formation in mouse femoral fracture model, suggesting that the combination of CB-bFGF with an collagen materials would promote vertebral fracture. Here, to attempt to apply the bone formation seeds to the vertebral fracute repair, we developed an injectable collagen powder (CP)/ CB-bFGF and demineralized bone powder (DBP)/CB-bFGF composite. CP/ CB-bFGF and BP/CB-bFGF composite accelerates bone formation in a vertebral bone defect model in rat and clearly showed that the composite also increases bone mineral density at fracture sites compared to CP and DBP alone. Our results suggest that CP/ CB-bFGF and DBP/CB-bFGF may be useful for promoting vertebral fracture healing in the clinical setting.

Free Research Field

運動器再生医療