2016 Fiscal Year Final Research Report
evaluation of organ protective effects of anesthetic agents using extra-cellular histone modification in liver ischemia-reperfusion injury
| Project/Area Number |
26861235
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Research Collaborator |
KAKIHANA Yasuyuki 鹿児島大学, 医歯学域医学系, 教授 (20264426)
YASUDA Tomotsugu 鹿児島大学, 医歯学域附属病院, 講師 (80437954)
ITOU Takashi 鹿児島大学, 医歯学総合研究科, 講師 (20381171)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 虚血再灌流障害 / ヒストン |
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| Outline of Final Research Achievements |
Ischemia-reperfusion injury occurs during a surgical procedure, such as liver resection, organ transplantation, and cardiovascular surgery. Moreover, it leads to severe tissue dysfunction and organ failure. Recent studies have shown that histones are released into the extracellular space during sepsis, trauma, and ischemia-reperfusion injury, and act as major mediators of the death of an organism. We investigated the correlation between extracellular histones and ischemia-reperfusion injury during liver resection and lower limb ischemia mouse model. This study showed the trends of the circulating histone H3 levels increase after ischemia-reperfusion and were related to ischemia time. We examined whether circulating histones are released from necrotic cells or neutrophils with cecal ligation and puncture(CLP)-induced sepsis. We also found that circulating histone H3 levels in septic conditions are mainly derived from neutrophils rather than damaged cells.
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| Free Research Field |
麻酔科学、集中治療医学
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