2016 Fiscal Year Final Research Report
The effect of minocycline on the neurotoxicity of general anesthetics in developmental brain.
Project/Area Number |
26861242
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Anesthesiology
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Research Institution | Nara Medical University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | トラマドール / Oデスメチルトラマドール / viability / アポトーシス / 細胞増殖能 |
Outline of Final Research Achievements |
We investigated whether minocycline and other treatment options can suppress the cytotoxicity of general or local anesthetics in the human neuroblastoma cell line SH-SY5Y. Contrary to our expectations, minocycline did not protect the cells against the viability-lowering effect of bupivacaine, however, morphine and necrostatine increased cell viability after the treatment with bupivacaine. In addition, we found that necrostatine itself possibly exhibited cytotoxicity. Next, we investigated the effects of tramadol and its active metabolite (O-desmethyltramadol) on the human lung cancer cell line H358. It was revealed that they decreased the viability of H358 cells and the underlying mechanism does not depend on promotion of cell apoptosis or inhibition of cell proliferation.
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Free Research Field |
麻酔科学
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