2015 Fiscal Year Final Research Report
The dynamic analysis of serum chemokines in the T-cell mediated rejection after renal transplantation and their application to new biomarker
| Project/Area Number |
26861269
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 腎移植 / 急性細胞性拒絶反応 / バイオマーカー / ケモカイン |
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| Outline of Final Research Achievements |
In the organ transplantation, it is important that I make an early diagnosis of T-cell mediated rejection (TCMR) non-invasively. We paid attention to chemokines. We made rat allograft model of renal transplantation and quantitative real-time PCR revealed that the expression of CCL2, CCL3, CCL5, CXCL9 and CXCL10 significantly was increased in the rat allograft from an early stage. In the human TCMR cases diagnosed by the graft biopsy, quantitative flow cytometry results showed that serum CXCL9, CXCL10 and CCL5 significantly elevated in comparison with the non-TCMR, and serum CXCL10 was suitable for a diagnosis prediction of TCMR by ROC curve. These results demonstrate that serum CXCL10 concentration may be a highly accurate biomarker for predicting TCMR.
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| Free Research Field |
腎移植
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