2016 Fiscal Year Final Research Report
Elucidation of novel molecular mechanisms in androgen independent growth of prostate cancer
| Project/Area Number |
26861280
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 前立腺癌 / miRNA / CRPC |
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| Outline of Final Research Achievements |
We are interested in molecular mechanisms involved in the progression of castration-resistant prostate cancer (CRPC). We found significantly higher expression of miR-30d in the androgen-independent LNCaP-AI cell line compared with the androgen-dependent LNCaP cell line using miRNA microarrays and qPCR. Clinicopathological study revealed that miR-30d expression levels were significantly higher in bone metastatic CRPC tissue than in untreated primary prostate cancer tissue. To evaluate the biological functions of miR-30d during the progression of CRPC, we determined whether miR-30d overexpressing cell lines grew well without androgen, and thus manifest growth characteristics of CRPC. As shown by the cell proliferation assay, LNCaP-30d cell lines were able to grow in an androgen-indepleted medium, while the cotrol cell (LNCaP-C) grew poorly in the absence of androgen. Taken together, we found that miR-30d promotes prostate cancer cell proliferation in an androgen-indepleted.
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| Free Research Field |
泌尿器病態学
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