Elucidation of development mechanism in renal cell carcinoma: focus on fatty acid receptor and visceral fat.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861297
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Keio University
• Principal Investigator: OBATA JUN 慶應義塾大学, 医学部, 助教 (20570865)
• Research Collaborator: MIYAJIMA AKIRA 慶應義塾大学, 医学部, 准教授 (90245572) ; OYA MOTOTSUGU 慶應義塾大学, 医学部, 教授 (00213885)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 脂肪酸受容体 / 肥満 / 腎細胞癌

Outline of Final Research Achievements

In renal cell carcinoma, we evaluated the impact of GPR120, a fatty acid receptor which has been identified as the important substance associated with metabolic syndrome. For examining short-term antitumor effect by stimulating GPR120, cell viability was measured subsequently after the exposure of agonists using WST-assay in a variety of renal cell carcinoma cell lines. We observed no obvious inhibition of proliferation and no change of GPR120 activity over time by western blot method. Although we also evaluated the long-term cytotoxicity by regular GPR120 stimulation, we found no remarkable change concerning the cellular proliferative potential and expression of GPR120 by western blot analysis regardless of conditions. Because it is expected that GPR120 is significantly associated with cancer for its anti-inflammatory effects, processes and results of our study under various conditions may be helpful for future study.

Free Research Field

医歯薬学