Changes in sperm perinuclear substance during fertilization

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861311
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: National Center for Child Health and Development (2015); Chiba University (2014)
• Principal Investigator: Yamatoya Kenji 国立研究開発法人国立成育医療研究センター, その他部局等, 研究員 (80447309)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 精子 / 受精 / 膜タンパク質

Outline of Final Research Achievements

To elucidate the mechanism that regulate the release of sperm DNA from the robust perinuclear structure during fertilization, we performed live imaging analysis of the processs, and mass spectrometric analysis to identify perinuclear substance related proteins. We identified several membrane proteins other than cytoskeletal proteins. Those membrane proteins under went post-translational modifications during capacitation. Live imaging analysis showed that perinuclear substance was peeled of from the sperm DNA and posttranslational modification of the perinuclear substance was needed for the process.

Free Research Field

生殖生物学