The therapeutic strategy for lung hypoplasia in congenital diaphragmatic hernia in a rat model

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861322
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nagoya University
• Principal Investigator: TSUDA HIROYUKI 名古屋大学, 医学部附属病院, 講師 (40571328)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 先天性横隔膜ヘルニア / 肺高血圧 / リモデリング

Outline of Final Research Achievements

Congenital diaphragmatic hernia (CDH) can induce lung hypoplasia and pulmonary hypertension, and is associated with high mortality. The purpose of this study is to examine the efficacy and safety of antenatal Saireito (TJ-114), a traditional Japanese herbal medicine, in a rat CDH model. Sprague-Dawley rats were exposed to an herbicide (nitrofen, 100 mg) on embryonic day 9 (E9) to induce CDH, and antenatal Saireito (2000 mg/kg/day) was orally administered from E10 to E20. On E21 fetuses were delivered. Antenatal Saireito significantly decreased the incidence of CDH (p < 0.01), and increased lung volume (p < 0.01), improved alveolarization and pulmonary artery remodeling using histological analysis, and respiratory function using gasometric analysis (pH; p < 0.05, and PCO2; p < 0.01). Taken together, our results demonstrated that antenatal Saireito can improve fetal pulmonary hypoplasia and pulmonary vascular remodeling, as a result, can improve respiratory function in a rat CDH model.

Free Research Field

周産期学