Identification of fetal-specific microRNAs as potential marker for amniotic fluid embolism

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861333
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nagasaki University
• Principal Investigator: HIGASHIJIMA Ai 長崎大学, 医歯薬学総合研究科(医学系), 助教 (00549595)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 産婦人科 / 羊水塞栓症 / microRNA / 母体血漿 / 胎児 / 胎盤 / C14MC

Outline of Final Research Achievements

Fifteen fetal blood cell-specific miRNAs were identified. Fourteen out of them located on chromosome 14 microRNA cluster region (C14MC; e.g. miR-370, miR-485, miR-432 etc), and remaining is miR-452 located on Xq-28. Micro RNAs on C14MC were highly expressed in cord blood cell, newborn blood cell and placental tissue, though microRNAs on C19MC were expressed only in placental tissue. By comprehensive analysis, microRNAs expressed highly in fetal blood cells than maternal blood cells were identified. Most of them located on C14MC region, and may be candidate molecular marker for amniotic fluid embolism. In addition, plasma concentrations of cell-free miR-323-3p could distinguish EP from SA yielding an area under the curve of 0.7454 (95% confidence interval: 0.5558-0.9349).

Free Research Field

産科婦人科学