2016 Fiscal Year Final Research Report
Combination of glucose uptake inhibition and radiation make synergistic inhibitory effect for head and neck squamous cell carcinoma
| Project/Area Number |
26861375
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 頭頸部癌 / FDG-PET |
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| Outline of Final Research Achievements |
We used human HNSCC cell lines. FaDu, Detroit562 and BICR6 had high expression of GLUT1, LDHA and MCT4. It revealed that anaerobic metabolism was high in these cell lines. Cell proliferation assay with or without glucose revealed that all of the cell lines without glucose grew slower than the cells with glucose. Glucose was essential for the growth of HNSCC cell lines. In hypoxia, FaDu, Detroit562 and BICR6 grew faster than those in normoxic condition. Western blot analyses revealed that the expressions of LDHA and MCT4 in hypoxia were higher than those in normoxia. In hypoxia, The knock down of GLUT1 or Ph-treatment inhibited the uptake of glucose. Cell proliferation assay revealed that HNSCC cell lines with Ph-treatment and radiation grew significantly slower than those with only radiation in hypoxia. Additionally, induction of apoptosis was detected in Ph-treated cell lines compared to cell lines treated with only radiation in hypoxia by TUNEL analysis.
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| Free Research Field |
頭頸部癌
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