2017 Fiscal Year Final Research Report
Protection of spiral ganglion cells by adenoviral mediated overexpression of Tis21 in rodent.
| Project/Area Number |
26861395
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kumamoto University |
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Principal Investigator |
Momoko Ise 熊本大学, 医学部附属病院, 助教 (20573596)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | Tis21遺伝子 / ラセン神経節細胞 / アデノウイルスベクター / 遺伝子導入による難聴治療 |
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| Outline of Final Research Achievements |
Our research plan was obliged to modify by technical issues. First, we injected the adenoviral vector into the round window in guinea pig not mouse, because it was difficult for us to inject into the round window in mouse. Second, deafening was demonstrated by gentamicin and ethacrynic acid not cisplatin because of our laboratory environment.Auditory thresholds at one month after deafening were protected by adenoviral mediated overexpression of Tis21, but not at two weeks. The number of spiral ganglion cells in treated group at two weeks and one month after deafening was maintained compared to that in non-treated group.
Our results showed Tis21 had an important role to protect spiral ganglion cells against drug-induced hearing loss in guinea pig
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| Free Research Field |
内耳基礎研究
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