Generation of tracheal chondrocytes from human iPS cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861405
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Fukushima Medical University
• Principal Investigator: Yoshie Susumu 福島県立医科大学, 医学部, 助教 (70705459)
• Research Collaborator: OMORI Koichi ; HAZAMA Akihiro ; WADA Ikuo ; IMAIZUMI Mitsuyoshi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヒトiPS細胞 / 気管 / 軟骨 / 再生

Outline of Final Research Achievements

The aim of this study is to regenerate tracheal defect by the use of an artificial trachea with human iPS cell-derived tracheal chondrocytes as a frame and collagen sponge as a scaffold. First, we could successfully induce human iPS cells into paraxial mesoderm with high efficiency using low molecular weight compounds and growth factors. Next, tracheal chondrocytes could be generated from human iPS cell-derived paraxial mesoderm by the overexpression of transcriptional factor A, which is related to chondrocyte differentiation. Finally, an artificial trachea with human iPS cell-derived tracheal chondrocytes and collagen sponge was implanted into tracheal defects in nude rats. At 14 d after implantation, the survaival of rat was confirmed. These results raised the possibility that our artificial trachea can regenerate tracheal defect.

Free Research Field

生理学