AP-1 family as a key molecule in the regulation of the pathways related to regional metastasis in head and neck squamous cell carcinoma

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861406
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Yokohama City University
• Principal Investigator: SANO Daisuke 横浜市立大学, 医学部, 助教 (10620990)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 頭頸部癌 / 遠隔転移 / AP-1

Outline of Final Research Achievements

We initially performed the upstream and key nodes analysis related with whole gene microarray analysis characterized by distant metastatic potential in vivo with HNSCC cell lines and identified JunB, member of the activated protein-1 (AP-1) family, as a key molecule regulating the pathways related with distant metastasis of HNSCC. Small interfering RNA (siRNA)-mediated knockdown of JunB, and Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (cas9) system (CRISPR/Cas9)-mediated knockout of JunB in HNSCC cells, markedly suppressed the ability of invasion and migration in vitro. In addition, knockout of JunB significantly repressed the incidence of lung metastases in vivo. These results suggested that JunB could play an important role to promote cell invasion, migration and distant metastasis of HNSCC.

Free Research Field

耳鼻咽喉科学