Functional analysis of the ChR expression ganglion cell by the retina slice patch clamp method

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861432
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tohoku University (2015); Iwate University (2014)
• Principal Investigator: Murayama Namie 東北大学, 大学病院, 産学官連携研究員 (60597516)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 眼生理 / パッチクランプ

Outline of Final Research Achievements

Channelrhodopsin, a molecular of light gated ion channel, is a promising therapeutic tool for vision restoration. Nonetheless, there are still few studies on the channelrhodopsin-mediated retinal neurotransmission. The purpose in this study is to investigate the response of the individual cell on the retinal slice by a patch clamp recordings.
We set up the method of the slice preparation and the slice patch clamp system. We made a construction of an adeno-associated virus vector (AAV-mGluR6-ChR2V) which specifically the target gene transduced into On-bipolar cells and could observe the gene expression in the On-bipolar cells on the cryo-sections. However, the transduction efficiencies into the On-bipolar cells was quite low, and it was difficult to find ChR2-expressing cells on the slice patch clamp recordings. We will try the patch clamp recordings on the retinal whole mount specimen.

Free Research Field

電気生理学