Development of corneal stromal reconstruction using biocompatible crosslinking agent and femtosecond laser

2017 Fiscal Year Final Research Report

• Project/Area Number: 26861447
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Osaka University
• Principal Investigator: SOMA Takeshi 大阪大学, 医学系研究科, 助教 (70582401)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 角膜実質 / 生体適合性架橋剤 / フェムト秒レーザー

Outline of Final Research Achievements

We developed a new treatment for corneal stromal thinning corneal stromal reconstruction using a biocompatible crosslinking agent composed of polyethylene glycol and model peptide and femtosecond laser in experimental rabbit. After horizontal incision was made to rabbit corneal stroma using a femtosecond laser, a mixture of a biocompatible crosslinking agent and type I collagen was injected into stromal pocket. An artificial stromal layer was confirmed in the middle layer of corneal stroma with a slit lamp microscope and an anterior ocular optical tomography postoperatively. In the histologic examination, an artificial stromal layer was also confirmed in the middle layer of the cornea. On the other hand, No invasion of blood vessels or inflammatory cells were observed at the injection site. Corneal stromal reconstruction using a new biocompatible crosslinking agent and femtosecond laser could be an effective treatment for thinning cornea.

Free Research Field

角膜移植、再生医療