investigation of molecular pathology of retinal detachment

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861459
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kagoshima University
• Principal Investigator: OTSUKA HIROKI 鹿児島大学, 医学部・歯学部附属病院, 医員 (20611817)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 分子生物学

Outline of Final Research Achievements

Retinal detachment is severe disease that can lead to loss of vision. Some patients have poor visual acuity by photoreceptor cell death, regardless of successful surgery. Recently, highly polarized RPE cells were grown on TranswellTM filters. We examined the pathophysiological mechanism of RPE and Thrombin using polarized RPE cells. Thrombin did not significantly affect the TER or the expression of tight-junctional proteins in polarized RPE cells, but decreased it in non-polarized RPE cells. Ki-67 positive cells were observed in non-polarized RPE cells but not in polarized RPE cells. Thrombin increases permeability of RPE in non-polarized RPE but not in polarized RPE.

Free Research Field

眼科学