2016 Fiscal Year Final Research Report
The Gene Function Analysis Using the Lympahangioma Cell Line
| Project/Area Number |
26861487
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Keio University |
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Principal Investigator |
Kato Motoyoshi 慶應義塾大学, 医学部(信濃町), 助教 (20573463)
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| Research Collaborator |
Fujino Akihiro
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | リンパ管腫 / リンパ管奇形 / 漢方薬 |
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| Outline of Final Research Achievements |
The purposes of this study were the elucidation of the mechanism for the pathogenesis of lymphangioma, the exploration of seeds for new therapeutic methods for the disease.
In this study, we established technique to make primary culture cells derived from lymphangioma lesions. And, we analyzed abnormally expressed genes, which we obtained from previous analysis of the cell, as a lymphangioma-related gene.
Recently, two Kampo medicines were reported to be effective for Lymphangimoma. We examined these drugs' direct effect on Lymphangioma derived cell. We co-cultured Lymphangioma cells with Kampo medicines and their crude drugs. They reduced proliferation rate, cell migration, and tube formation. Then, permeability of the cell sheet was increased. Our basic study supported that Kampo medicine could be a therapeutic option for lymphangioma.
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| Free Research Field |
小児外科
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