Analysis of the dedifferentiation process of chondrocytes using Time-lapse observation with new lineage tracing technique

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861496
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: University of Fukui
• Principal Investigator: Minegishi Yoshiki 福井大学, 学術研究院医学系部門(附属病院部), 助教 (10467566)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 軟骨細胞 / 脱分化 / lineage tracing

Outline of Final Research Achievements

When chondrocytes prepared from cartilage are expanded in monolayer culture, fibroblast-like cells gradually prevail. Although these prevailing fibroblast-like cells are believed to emerge because of the dedifferentiation of chondrocytes, the definite origin of the prevailing fibroblast-like cells has not been determined. We herein examined whether the prevailing non-chondrocytic cells observed after monolayer expansion culture arise from dedifferentiating chondrocytes or are the result of the overgrowth of fibroblasts that are present at the start of the culture. We also evaluated whether chondrocytes dedifferentiate because they proliferate or because they are cultured in monolayers.

Free Research Field

軟骨細胞