Clarification of cell biological properties of diabetic adipose tissue derived stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861513
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: SUDA Shunichi 順天堂大学, 医学部, 非常勤助手 (70645861)
• Research Collaborator: TANAKA Rica 順天堂大学, 医学部, 准教授 (70509827) ; TOBITA Morikuni 順天堂大学, 医学部, 准教授 (10599038) ; MIZUNO Hiroshi 順天堂大学, 医学部, 教授 (80343606)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 形成外科 / 幹細胞 / 脂肪組織 / 血管内皮細胞

Outline of Final Research Achievements

As diabetic patients increase, the patients suffering from non-healing diabetic ulcer also increases and many of them go under lower limb amputations. There is no effective treatment for these patients after treatment modalities are exhausted. One of the etiologies is dysfunction of endothelial progenitor cells (EPC) which is the vascular stem cells in diabetic patients. Here, we focused the functions of adipose tissue-derived stem cells (ASC) in diabetes. We found that ASC were difficult to differentiate to vascular endothelial cells. On the other hands, ASC secreted various angiogenic factors to enhance angiogenesis. However, these founding were decreased in diabetes. ASC enhanced the wound closure and increased the viability of transplanted adipose tissue when mixing with EPC in mice. From these results, we conclude that ASC show strong indirect effects of angiogenesis through the secretion of angiogenic factors and the function is enhanced with co-transplantation with EPCs.

Free Research Field

形成外科学