2017 Fiscal Year Final Research Report
Development of blood vessel and skin regeneration therapy using Adipose-derived Stem Cells and hypoxic preconditioning
| Project/Area Number |
26861515
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 脂肪幹細胞 / 低酸素 / 増殖 |
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| Outline of Final Research Achievements |
Adipose tissue-derived stem cells (ASCs) have been recently isolated from human subcutaneous adipose tissue. ASCs may be useful in regenerative medicine as an alternative to bone marrow-derived stem cells. Changes in the oxygen concentration influence physiological activities, such as stem cell proliferation. However, the effects of the oxygen concentration on ASCs remain unclear. In the present study, the effects of hypoxia on ASC proliferation were examined.Normal human adipose tissue was collected from the lower abdomen, and ASCs were prepared with collagenase treatment. ASC proliferation was significantly enhanced in the hypoxic culture and was inhibited by ERK and Akt inhibitors. Hypoxia for 5-15 minutes stimulated the phosphorylation of ERK1/2 among MAP kinases and induced HIF-1α expression.
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| Free Research Field |
形成外科
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