miRNAs controlling endoplasmic reticulum stress in monocytes in a sepsis model

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861527
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Ishii Sachiyo 京都府立医科大学, 医学部附属病院, 専攻医 (40457958)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: micro RNA / 敗血症 / 小胞体ストレス

Outline of Final Research Achievements

In the sepsis model used, cultured monocytes and human macrophages were treated with LPS, leading to time-based changes in their phagocytic abilities and in the mRNA and protein levels of CHOP, which is an endoplasmic reticulum stress related molecule. Moreover, we observed altered expression of miR-21 using the Ion PGM system for next-generation sequencing, suggesting that the over-expression or downregulation of miRNAs by gene transfection might induce changes in phagocytic abilities. Currently, we are in the process of examining the effect of miRNA transfection on endoplasmic reticulum stress, and in future, plan to study the effect in a rat sepsis model.

Free Research Field

集中治療医学、麻酔科学