• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2016 Fiscal Year Final Research Report

Developing a novel biological chamber system for oral bacterial infection.

Research Project

  • PDF
Project/Area Number 26861568
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionTohoku University

Principal Investigator

Matsui Aritsune  東北大学, 大学病院, 医員 (60547264)

Project Period (FY) 2014-04-01 – 2017-03-31
Keywords歯性感染症 / 免疫応答
Outline of Final Research Achievements

We established an efficient biological design for the bacterial infection model; cylinder shaped chamber made of Poly dimethyl siloxane (PDMS), 100-200 um sized pores were added for immune cells infiltration into the device. Immunological investigation (e.g. Flowcytometry) for the collected cells and histomorphometric examination of biofilm formation which formed inside of the chamber were performed. The device showed an advantage especially for the analysis of neutrophil. Mixture of 4 common oral bacterial species ((P. intermedia (ATCC 25611), F. nucleatum (ATCC 25586), P. micros (ATCC 33270), S. intermedius (ATCC 27335)) showed thicker biofilm formation compared with the control species (Streptococcus mitis) at 24h time point after the infection. Surface preparation of the PDMS against for the exfoliation of biofilm formation seemed necessary for further structural analyses.

Free Research Field

免疫学

URL: 

Published: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi