2014 Fiscal Year Research-status Report
高齢者インフルエンザ感染予防における歯肉塗布ワクチンジェルの開発
| Project/Area Number |
26861580
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| Research Institution | Nihon University |
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Principal Investigator |
CUENO Marni 日本大学, 歯学部, ポスト・ドクトラル・フェロー (20569967)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | ワクチンジェル / インフルエンザ / 高齢者ワクチン |
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| Outline of Annual Research Achievements |
Objective: To determine the optimal conditions and concentrations for gel-encapsulation and application along the gingival crevice.
Methods: Xanthan gel was used to encapsulate all samples. Catechin component was initially used for preliminary comparison and optimization to be cost-effective. Throughout this year, we used 20 wk-old (young), 40 wk-old (middle-age), and 77 wk-old (elderly) Sprague-Dawley rats. In order to confirm the age-related difference in the gingival crevice, we compared catechin entry to the body via oral-administration and oral-supplementation. Subsequently, we determine the ideal xanthan gel:catechin ratio and optimal gel-encapsulated catechin concentration that would allow the highest amount to enter the body. Confirmation was done using HPLC.
Results: We found higher O-methyl levels among rats with orally-supplemented catechin as compared to orally-administered catechin. Moreover, we observed that higher catechin amounts enter the body of elderly rats as compared to the other age groups. We observed that O-methyl levels in the blood sera were highest at 100 μg/mL catechin concentration which would imply that this is the optimum amount for oral-supplementation.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We were able to follow our set yearly plan and objectives. Moreover, first year results were consistent with our first year objectives. In addition, we were able to publish our preliminary data in a reputable journal.
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| Strategy for Future Research Activity |
Objective: To confirm whether xanthan gel-encapsulation would interfere with antibody epitopes and to test whether oral-supplementation of antigens along the gingival mucosa can induce an immune response.
Methods: Influenza H5N1 hemagglutinin (HA) will be used as a representative antigen and 77 wk-old (elderly) Sprague-Dawley rats will be used throughout this study. Initially, molecular docking of the xanthan gel on the HA crystal structure will be performed in order to determine whether antibody epitopes are blocked. Subsequently, optimal conditions and concentration previously established in the first year will be used to formulate a gel-encapsulated HA (GEH) which would be orally-supplemented along the upper and lower molars in order to vaccinate the rat. Moreover, intradermal, oral, and sublingual vaccinations will be performed to assess the effectiveness of gingival vaccination via oral-supplementation. Antibody titer produced after vaccination will be measured through ELISA.
Expected results: (1) Xanthan gel encapsulation will not inhibit HA antibody epitopes; and (2)Gingival vaccination via oral-supplementation would stimulate an antibody response comparable to other vaccination strategies.
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| Causes of Carryover |
We were able to optimize the conditions and concentrations for gel vaccine development efficiently. Thus, for the first year of the study, we were able to save some amount.
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| Expenditure Plan for Carryover Budget |
We plan to add the excess money from the first year to the second year budget. We expect that the second year of study would be more costly compared to the first year since we will need to optimize the conditions for measuring the neutralizing antibody produced. Thus, the extra amount would help with the proposed expenses.
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Research Products
(4 results)
