The interplay between bone and ex vivo-expanded gamma delta T cells.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861583
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Osaka Dental University
• Principal Investigator: DOMAE Eisuke 大阪歯科大学, 歯学部, 講師 (50454559)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: γδ T cells / IL-12 / IL-18 / STAT4 / NF-κB / IκBζ

Outline of Final Research Achievements

We examined the effects of inflammatory environment on ex-vivo expanded Vγ9Vδ2 T cells. Vγ9Vδ2 T cells treated with IL-12 and IL-18 enhanced effector functions, including the expression of IFN-γ and granzyme B, and cytotoxicity. These enhanced effector responses following IL-12 and IL-18 treatment were associated with homotypic aggregation, enhanced expression of ICAM-1 and decreased expression of the B- and T-lymphocyte attenuator (BTLA), a co- inhibitory receptor. IL-12 and IL-18 also induced the antigen-independent proliferation of Vγ9Vδ2 T cells. Increased expression of IκBζ, IL-12Rβ2 and IL-18Rα following IL-12 and IL-18 stimulation resulted in sustained activation of STAT4 and NF-κB. Thus, we showed that IL-12 and IL-18 synergize to activate human ex vivo-expanded Vγ9Vδ2 T cells.

Free Research Field

生化学、免疫学