Development of the multi-shell nanohydroxyapatite-collagen scaffold with control system of releasing growth factor

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861667
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tohoku University
• Principal Investigator: Tenkumo Taichi 東北大学, 歯学研究科(研究院), 助教 (80451425)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ナノハイドロキシアパタイト / リン酸カルシウム / 遺伝子導入 / 生体材料 / プロタミン

Outline of Final Research Achievements

Fundamental elements of tissue regeneration include cells, growth factors, and a scaffold. This study aimed to fabricate a growth factor releasing biodegradable scaffold for tissue regeneration. We prepared multi-shell calcium phosphate (CaP) nanoparticles functionalised with DNA, polyethyleneimine (PEI), protamine, and octa-arginine (R8), and compared their respective transfection activity and cell viability measures using human mesenchymal stem cells. These experiments demonstrated that the scaffolds were associated with moderate hMSC cell viability and were capable of releasing the BMP-2 protein into hMSCs following gene transfection. In particular, the scaffold loaded with protamine-containing CaP nanoparticles demonstrated the highest cell viability and transfection efficiency in hMSCs; thus, it might be suitable to serve as an efficient growth factor releasing scaffold.

Free Research Field

歯周組織再生