Application of direct reprogramming technology to periodontal tissue Regenerative therapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861679
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Yamamoto Kenta 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00636160)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 再生医療 / 骨芽細胞 / ダイレクトリプログラミング

Outline of Final Research Achievements

Alveolar bone resorption associated with advanced periodontitis causes tooth loss to remarkably reduce patients’ QOL. Transplantation of autologous osteoblasts into the alveolar bone legion may facilitate alveolar bone regeneration to prevent tooth loss. Recently we reported that human fibroblasts were directly reprogrammed into osteoblasts by transducing four transcription factor genes by means of retrovirus vectors. In this study, we improved this technology to obtain reprogrammed osteoblasts in which any transgene is not integrated into their chromosomes.
As results, human fibroblasts were successfully converted into osteoblasts by transfecting genes by a plasmid vector. Transgenes were not integrated into the chromosome of the resultant cells. The present technology may lead to novel regenerative medicine for alveolar bone resorption.

Free Research Field

再生歯科医学、骨免疫学、歯周病学