effect of peripheral blood mononuclear cells and beta-tricalcium phosphate on bone regeneration

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861683
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Meikai University
• Principal Investigator: Ishii Makiko 明海大学, 歯学部, 助教 (00637986)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 歯槽骨再生 / 骨再生 / 歯周組織再生療法 / β-TCP / 末梢血由来単核球細胞 / 不織布フィルター

Outline of Final Research Achievements

We aimed to develop a novel bone grafting material. We isolated peripheral blood mononuclear cells (PBMCs) from venous blood taken from the front legs of male mongrel adult dogs by means of gradient centrifugation (Ficoll group) or nonwoven filtering (Filter group), and mixed and incubated these cells with β-TCP microgranules to adhere to the surface of β-TCP granules. We then grafted the PBMCs/β-TCP granules into experimentally prepared bone defects in dog mandibles. A tendency toward greater osteogenesis was recognized in the Ficoll and Filter groups compared with the control group. Furthermore, the procedure for the filtering method is simple and may be applicable for dental graft treatment in general dental clinics. Taken together, PBMCs harvested using a nonwoven filter and incubated with β-TCP granules may provide a useful material for regenerating periodontal tissue and bone.

Free Research Field

歯周病学