2016 Fiscal Year Final Research Report
Effect of transcriptional factor Alx3 during osteoblast differentiation and bone development
| Project/Area Number |
26861684
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Alx3 / BMP |
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| Outline of Final Research Achievements |
In this study, we demonstrated the mechanisms of Alx3 gene expression and function during osteoblast differentiation induced by BMP-2. In C2C12 cells, BMP-2 induced increase of Alx3 gene expression in both time- and dose-dependent manners through the Smad signaling pathway. In addition, silencing of Alx3 br siRNA inhibited osteoblast differentiation induced by BMP-2, as showed by the expressions of Alkaline phosphatase (ALP), Osteocaltin, and Osterix, while overexpression of Alx3 enhanced osteoblast differentiation induced by BMP-2. According to Alx3 CHIP assay, osteoblastic marker gene ALP is the target gene of Alx3 transcriptional factor. These results indicate that Alx3 is a positive regurator of osteoblast differentiation induced by BMP-2. However, we found that effect of osteoblast diffarentiation by Alx3 is not enough and therefore Alx3 applied bone development is dificult.
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| Free Research Field |
歯学
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