2015 Fiscal Year Final Research Report
Generation and analysis of oral and maxillofacial disease-specific human iPSCs in completely serum-, feeder-, and integration-free culture
| Project/Area Number |
26861723
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 歯学 / 再生医学 / 幹細胞 / 遺伝子疾患 |
|---|
| Outline of Final Research Achievements |
Human pluripotent stem cells hold great promise for their practical and scientific potentials. To improve understanding of self-renewal and differentiation, we could generate and maintain human iPSCs in serum- and feeder-free culture conditions using retroviral vectors. To avoid the unpredictable side effects associated with retrovirus integration, we generated hiPSCs from dental pulp cells and PBMCs with a non-integrating replication-defective and persistent Sendai virus (SeVdp) vector. Using this system, pluripotent and self-renewing hiPSCs could be easily and stably generated and propagated. With this system we established hiPSCs from cleidocranial dysplasia (CCD) caused by a mutation of RUNX2, which has an important role in the differentiation of osteoblasts and maturation of chondrocytes. The cartilage in the teratomas of CCD-iPSCs showed abnormalities. These CCD-iPSCs would be beneficial to clarify the molecular mechanism and for development of medical applications.
|
| Free Research Field |
外科系歯学
|
