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2015 Fiscal Year Final Research Report

Regulation of metabotropic glutamate receptor 5 expression by the miR-30 downregulation induced by the SDF-1/CXCR4 system in oral cancer cells.

Research Project

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Project/Area Number 26861725
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionDokkyo Medical University (2015)
The University of Tokushima (2014)

Principal Investigator

Kuribayashi Nobuyuki  獨協医科大学, 医学部, 助教 (80617332)

Project Period (FY) 2014-04-01 – 2016-03-31
KeywordsmicroRNA / mGluR5 / CXCR4
Outline of Final Research Achievements

In this study, we examined the miRNA association involved in the mGluR5 expression using oral cancer cells, B88, which express functional CXCR4 and exhibit highly metastatic potentials. We examined the metastasis-related miRNAs in SDF-1 stimulated B88 cells by use of a miRNA microarray analysis. Consequently, we isolated miR-30 family which has predictive binding sites in 3'-UTR of mGluR5 mRNA in silico analysis. Among these miRNAs, we confirmed the downregulation of all miR-30 family in SDF-1 stimulated B88 cells by the real-time PCR analysis. Next, we transfected these miR-30 families in SDF-1 stimulated B88 cells. We confirmed the downregulation of mGluR5 by the miR-30a-5p and miR-30c-5p overexpression. These results indicated that SDF-1/CXCR4 system might regulate the metastases of oral cancer by the upregulation of mGluR5 via downregulation of miR-30 family.

Free Research Field

外科系歯学

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Published: 2017-05-10  

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